Antigens & Antibodies for Diagnostic Kit Development
 
   Committed to high sensitivity & specificity

Background

 

The current methods of detectinga SARS-CoV-2 infection include nucleic acid detections, antibody detections, and antigen detections. Compared to nucleic acid detections, antibody detections and antigen detections are faster and more convenient. Therefore, they are suitable for scenarios such as rapid screening and instant detection.

The sensitivity and specificity of the serological diagnostic kits are significantly affected by the quality of antigen and antibody reagents. To better support the development of the diagnostic kits, ACROBiosystems has developed a serial of high-quality antigen and antibody reagents. Furthermore, several in vitro diagnostics companies are using ACRO’s reagents to develop COVID-19 serological diagnostics products. Among them, Babson Diagnostics, a US diagnostics company, successfully obtained the Emergency Use Authority from FDA and launched their chemiluminescent COVID-19 antibody detection kit employing ACRO’s biotin-labeled S1 protein.

Reagents for antigen test

Reagents for neutralizing antibody

Reagents of IgG/IgM antibody

Reagents for antigen test

ACROBiosystems has launched matched antibody pairs that are specifically designed and screened for antigen detection kit development, which are suitable for different diagnostic assays, such as chemiluminescent immunoassay, ELISA, gold immunochromatography assay, etc. The sensitivity of the sandwich ELISA can be as low as 12 pg/mL in the verification study using recombinant antigens. In addition, the antibody pairs have been verified using inactivated virus samples. The titer, which was determined using our LFA assay, is as low as 5 TCID50.

Facing increasingly complex mutations, scientists are concerned whether the new mutants could be detected by rapid antigen tests. ACROBiosystems successfully verified that our high-sensitive antibody pair (Cat. No. S1N-M12A1/S1N-M13A1) can detect the latest SARS-CoV-2 variants.

Product list

CategoryCat. No.SpeciesProduct Description
Nucleocapsid proteinNUN-S91MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS91) (Trehalose free)
NUN-S92MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS92) (Trehalose free)
NUN-S46MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (Trehalose free)
NUN-S47MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) (Trehalose free)
NUN-S57MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS57) (Trehalose free)
NUN-S60MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS60) (Trehalose free)
NUN-S61MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS61) (Trehalose free)
S1 proteinS1N-M12A1MouseAnti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free)HOT
S1N-M13A1MouseAnti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Trehalose free)HOT
S1N-S58MouseAnti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS58) (Trehalose free)
S1N-S59MouseAnti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59) (Trehalose free)

These antibodies were recommended for antigen detection by double antibody sandwich.

>>>  Virus lysis buffer (Cat.No. LY-13) is available for antigen detection kit development.
>>>  If you have any bulk order inquiry, please feel free to click here.

Reagents for neutralizing antibody

Testing the levels of neutralizing antibodies against S protein in serum samples is critical in determining the efficacy of vaccines as well as a diagnostic measure of past infections. With the beginning of large-scale immunization with multiple vaccines and the rise of mutant variants, close monitoring of neutralizing antibodies in population and the impact of virus mutation is necessary for effective immunization campaign. ACROBiosystems has developed multiple high quality RBD proteins and ACE2 proteins to support neutralizing antibody test development. ACROBiosystems has developed multiple high quality RBD proteins and ACE2 proteins to support neutralizing antibody test development.

Product list

CategoryCat. No.Product description
ACE2 proteinAC2-H5255Human ACE2 / ACEH Protein, Fc Tag (MALS verified)
AC2-H82E7Biotinylated Human ACE2 / ACEH Protein, His,Avitag™
RBD proteinSPD-C5251SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag
SPD-C5255SARS-CoV-2 (COVID-19) S protein RBD, Fc Tag (MALS verified)
SPD-C52M5SARS-CoV-2 S protein RBD trimer, His Tag (MALS verified)
Neutralizing antibodyS1N-M12A1Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free)
SPD-M180Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (AM180)
SPD-M128Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128)

Product list

MoleculeCat. No.HostTagRecommended applicationRecommended method
S proteinSPN-C52H7HEK293His TagDetect IgG/IgM by capture methodELISA
GICA
CLIA
S1 proteinS1N-C52H2HEK293His TagDetect IgG/IgM by capture method
S1N-C82E9HEK293His,Avi tag™Detect IgG/IgM by capture method
S2 proteinS2N-C52H2HEK293His TagDetect IgG/IgM by capture method
RBD proteinSPD-C52H1HEK293His TagDetect total antibodies by double antigen sandwich method (detection)
SPD-C52H2HEK293His TagDetect IgG/IgM by capture method
SPD-C5251HEK293Mouse Fc TagDetect total antibodies by double antigen sandwich method (capture)
SPD-C82E8HEK293His,Avi tag™Detect IgG/IgM by capture method
Nucleocapsid proteinNUN-C5221HEK293His TagDetect IgG/IgM by capture method
NUN-C51H2E.coliHis TagDetect IgG/IgM by capture method
>>>  If you have any bulk order inquiry, please feel free to click here.

>>>  If you have any custom inquiries or suggestions for SARS-CoV-2 (COVID-19) related products, please click here.

IgG/IgM control antibodies are available for antibody detection kit development.

Cat. No.SpeciesProduct DescriptionSubtypeMolecule
S1N-M122MouseAnti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb (AM122)Human IgG1S protein RBD
SPD-M180HumanAnti-SARS-CoV-2 RBD Potent Neutralizing Antibody (AM180)Human IgG1S protein RBD
SAD-S35HumanAnti-SARS-CoV-2 Spike RBD Neutralizing Antibody (AS35)Human IgG1S protein RBD
SPD-M128MouseAnti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb (AM128)Human IgG1S protein RBD
S1N-M130MouseAnti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb (AM130)Human IgG1S protein RBD
SPD-M121MouseAnti-SARS-CoV-2 Spike NTD Neutralizing Antibody, Chimeric mAb (AM121)Human IgG1S1 protein NTD
SPD-M162MouseAnti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb (AM122)Human IgMS protein RBD
S2N-S86HumanAnti-SARS-CoV-2 Spike S2 Antibody (AS86)Human IgG4S2 protein